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b85502 hek293t cell line atcc  (ATCC)


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    Structured Review

    ATCC b85502 hek293t cell line atcc
    B85502 Hek293t Cell Line Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 38021 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/b85502+hek293t+cell+line+atcc/293T/pm39093700-646-199-203
    Average 99 stars, based on 38021 article reviews
    b85502 hek293t cell line atcc - by Bioz Stars, 2026-09
    99/100 stars

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    Virus:

    Article Title: Structural insights into ligand recognition and activation of the succinate receptor SUCNR1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC-labeled anti-FLAG antibody Sigma Cat# F4049; RRID: AB_439701 Bacterial and virus strains E. coli DH5a NEB Cat# C2988 Chemicals, peptides, and recombinant proteins Apyrase Sigma Cat# A6535 Protease Inhibitor Cocktail, EDTA-free TargetMol Cat# C0001 n-dodecyl-b-d-maltoside (DDM) Anatrace Cat# D310 Cholesteryl hemisuccinate (CHS) Anatrace Cat# CH210 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432 Lauryl maltose neopentyglycol (LMNG) Anatrace Cat# NG310 High Affinity Ni-Charged Resin FF GenScript Biotech Cat# L00666 SIM SF Expression Medium SinoBiological Cat# MSF1 DMEM Gibco Cat# 11965092 Characterized Fetal Bovine Serum (FBS), New Zealand Origin HyClone Cat# SH30406.05 HBSS, Calcium, magnesium Gibco Cat# 14025-092 Succinate Sangon Biotech Cat# A610496 cis-epoxysuccinate MCE Cat# HY-125791 FLAG peptide GenScript Custom Synthesis Lipofectamine 3000 Invitrogen Cat# L3000001 Forskolin MCE Cat# HY-15371 Coelenterazine H Yeasen Biotech Cat# 40906ES Critical commercial assays ClonExpress Ultra One Step Cloning Kit Vazyme Biotech Cat# C115 Bac-to-Bac Expression System Invitrogen Cat# A11098 LANCE Ultra cAMP kit PerkinElmer Life Sciences Cat# TRF0263 Deposited data succinate-SUCNR1-Gi complex EM map This work EMDB entry ID: EMD-37686 succinate-SUCNR1-Gi complex coordinates This work PDB entry ID: 8WOG epoxysuccinate-SUCNR1-Gi complex EM map This work EMDB entry ID: EMD-37707 epoxysuccinate-SUCNR1-Gi complex coordinates This work PDB entry ID: 8WP1 Experimental models: Cell lines Spodoptera frugiperda Sf9 insect cell line Invitrogen Cat# 11496015 Trichoplusia ni Hi5 insect cell line Invitrogen Cat# B85502 HEK293T cell line ATCC Cat# CRL-3216 HeLa cell line ATCC Cat# CCL2 Recombinant DNA pFastBac-FLAG-SUCNR1 This work N/A pFastBac-DN Gai This work N/A pFastBac-Gb1 This work N/A pFastBac-Gg2 This work N/A pcDNA3.1-SUCNR1 This work N/A pcDNA3.1-SUCNR1 (Y30A) This work N/A (Continued on next page) Cell Reports 43, 114381, July 23, 2024 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1-SUCNR1 (Y83A) This work N/A pcDNA3.1-SUCNR1 (R99A) This work N/A pcDNA3.1-SUCNR1 (F175A) This work N/A pcDNA3.1-SUCNR1 (Y248A) This work N/A pcDNA3.1-SUCNR1 (Y277A) This work N/A pcDNA3.1-SUCNR1 (R281A) This work N/A pcDNA3.1-Gai1-LgBiT This work N/A pcDNA3.1-Gb1 This work N/A pcDNA3.1-SmBiT-Gg2 This work N/A Software and algorithms cryoSPARC v3.3.1 Structura Biotechnology https://cryosparc.com/ Chimera UCSF https://www.cgl.ucsf.edu/chimera/ ChimeraX UCSF https://www.cgl.ucsf.edu/chimerax/ COOT Emsley et al., 201036 https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Phenix Liebschner et al., 201937 https://phenix-online.org/ CHARMM-GUI Lehigh University https://www.charmm-gui.org/ GROMACS Abraham et al., 201538 https://www.gromacs.org/ PyMOL Schrödinger https://pymol.org/2/ GraphPad Prism GraphPad https://www.graphpad.com/ Other Amicon Ultra Centrifugal Filters-100k Millipore Cat# UFC9100 Superose 6 Increase 10/300GL column GE Healthcare Cat# 29091596 Superdex 200 Increase 10/300 column GE Healthcare Cat# 28990944 Quantifoil Au 300 mesh R1.2/1.3 grids Sigma Cat# TEM-Q350AR1.3

    Article Title: Cryo-EM structure of monomeric CXCL12-bound CXCR4 in the active state.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APC-labeled anti-CXCR4 antibody Thermo Fisher Scientific Cat# 17-9999-42; RRID:AB_1724113 Bacterial and virus strains E. coli DH5a NEB Cat# C2988 Chemicals, peptides, and recombinant proteins Apyrase Sigma Cat# A6535 Protease Inhibitor Cocktail, EDTA-free TargetMol Cat# C0001 n-dodecyl-b-d-maltoside (DDM) Anatrace Cat# D310 Cholesteryl hemisuccinate (CHS) Anatrace Cat# CH210 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432 Lauryl maltose neopentyglycol (LMNG) Anatrace Cat# NG310 High Affinity Ni-Charged Resin FF GenScript Biotech Cat# L00666 SIM SF Expression Medium SinoBiological Cat# MSF1 DMEM Gibco Cat# 11965092 Characterized Fetal Bovine Serum (FBS), New Zealand Origin HyClone Cat# SH30406.05 HBSS, Calcium, magnesium Gibco Cat# 14025-092 Human CXCL12 PeproTech Cat# 30028A FLAG peptide GenScript Custom Synthesis Lipofectamine 3000 Invitrogen Cat# L3000001 Forskolin MCE Cat# HY-15371 Coelenterazine H Yeasen Biotech Cat# 40906ES Critical commercial assays ClonExpress Ultra One Step Cloning Kit Vazyme Biotech Cat# C115 Bac-to-Bac Expression System Invitrogen Cat# A11098 LANCE Ultra cAMP kit PerkinElmer Life Sciences Cat# TRF0263 Deposited data CXCL12-CXCR4-Gi complex EM map This work EMDB entry ID: EMD-36869 CXCL12-CXCR4-Gi complex coordinates This work PDB entry ID: 8K3Z Experimental models: Cell lines Spodoptera frugiperda Sf9 insect cell line Invitrogen Cat# 11496015 Trichoplusia ni Hi5 insect cell line Invitrogen Cat# B85502 HEK293T cell line ATCC Cat# CRL-3216 HeLa cell line ATCC Cat# CCL2 Recombinant DNA pFastBac-FLAG-CXCR4 This work N/A pFastBac-DN Gai This work N/A pFastBac-Gb1 This work N/A pFastBac-Gg2 This work N/A pFastBac-CXCL12 This work N/A pFastBac-gp67-scFv16 This work N/A pcDNA3.1-CXCR4 This work N/A pcDNA3.1-CXCR4(C28A) This work N/A pcDNA3.1-CXCR4(R134A) This work N/A (Continued on next page) Cell Reports 43, 114578, August 27, 2024 15 ..

    Recombinant:

    Article Title: Structural insights into ligand recognition and activation of the succinate receptor SUCNR1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC-labeled anti-FLAG antibody Sigma Cat# F4049; RRID: AB_439701 Bacterial and virus strains E. coli DH5a NEB Cat# C2988 Chemicals, peptides, and recombinant proteins Apyrase Sigma Cat# A6535 Protease Inhibitor Cocktail, EDTA-free TargetMol Cat# C0001 n-dodecyl-b-d-maltoside (DDM) Anatrace Cat# D310 Cholesteryl hemisuccinate (CHS) Anatrace Cat# CH210 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432 Lauryl maltose neopentyglycol (LMNG) Anatrace Cat# NG310 High Affinity Ni-Charged Resin FF GenScript Biotech Cat# L00666 SIM SF Expression Medium SinoBiological Cat# MSF1 DMEM Gibco Cat# 11965092 Characterized Fetal Bovine Serum (FBS), New Zealand Origin HyClone Cat# SH30406.05 HBSS, Calcium, magnesium Gibco Cat# 14025-092 Succinate Sangon Biotech Cat# A610496 cis-epoxysuccinate MCE Cat# HY-125791 FLAG peptide GenScript Custom Synthesis Lipofectamine 3000 Invitrogen Cat# L3000001 Forskolin MCE Cat# HY-15371 Coelenterazine H Yeasen Biotech Cat# 40906ES Critical commercial assays ClonExpress Ultra One Step Cloning Kit Vazyme Biotech Cat# C115 Bac-to-Bac Expression System Invitrogen Cat# A11098 LANCE Ultra cAMP kit PerkinElmer Life Sciences Cat# TRF0263 Deposited data succinate-SUCNR1-Gi complex EM map This work EMDB entry ID: EMD-37686 succinate-SUCNR1-Gi complex coordinates This work PDB entry ID: 8WOG epoxysuccinate-SUCNR1-Gi complex EM map This work EMDB entry ID: EMD-37707 epoxysuccinate-SUCNR1-Gi complex coordinates This work PDB entry ID: 8WP1 Experimental models: Cell lines Spodoptera frugiperda Sf9 insect cell line Invitrogen Cat# 11496015 Trichoplusia ni Hi5 insect cell line Invitrogen Cat# B85502 HEK293T cell line ATCC Cat# CRL-3216 HeLa cell line ATCC Cat# CCL2 Recombinant DNA pFastBac-FLAG-SUCNR1 This work N/A pFastBac-DN Gai This work N/A pFastBac-Gb1 This work N/A pFastBac-Gg2 This work N/A pcDNA3.1-SUCNR1 This work N/A pcDNA3.1-SUCNR1 (Y30A) This work N/A (Continued on next page) Cell Reports 43, 114381, July 23, 2024 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1-SUCNR1 (Y83A) This work N/A pcDNA3.1-SUCNR1 (R99A) This work N/A pcDNA3.1-SUCNR1 (F175A) This work N/A pcDNA3.1-SUCNR1 (Y248A) This work N/A pcDNA3.1-SUCNR1 (Y277A) This work N/A pcDNA3.1-SUCNR1 (R281A) This work N/A pcDNA3.1-Gai1-LgBiT This work N/A pcDNA3.1-Gb1 This work N/A pcDNA3.1-SmBiT-Gg2 This work N/A Software and algorithms cryoSPARC v3.3.1 Structura Biotechnology https://cryosparc.com/ Chimera UCSF https://www.cgl.ucsf.edu/chimera/ ChimeraX UCSF https://www.cgl.ucsf.edu/chimerax/ COOT Emsley et al., 201036 https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Phenix Liebschner et al., 201937 https://phenix-online.org/ CHARMM-GUI Lehigh University https://www.charmm-gui.org/ GROMACS Abraham et al., 201538 https://www.gromacs.org/ PyMOL Schrödinger https://pymol.org/2/ GraphPad Prism GraphPad https://www.graphpad.com/ Other Amicon Ultra Centrifugal Filters-100k Millipore Cat# UFC9100 Superose 6 Increase 10/300GL column GE Healthcare Cat# 29091596 Superdex 200 Increase 10/300 column GE Healthcare Cat# 28990944 Quantifoil Au 300 mesh R1.2/1.3 grids Sigma Cat# TEM-Q350AR1.3

    Article Title: Cryo-EM structure of monomeric CXCL12-bound CXCR4 in the active state.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APC-labeled anti-CXCR4 antibody Thermo Fisher Scientific Cat# 17-9999-42; RRID:AB_1724113 Bacterial and virus strains E. coli DH5a NEB Cat# C2988 Chemicals, peptides, and recombinant proteins Apyrase Sigma Cat# A6535 Protease Inhibitor Cocktail, EDTA-free TargetMol Cat# C0001 n-dodecyl-b-d-maltoside (DDM) Anatrace Cat# D310 Cholesteryl hemisuccinate (CHS) Anatrace Cat# CH210 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432 Lauryl maltose neopentyglycol (LMNG) Anatrace Cat# NG310 High Affinity Ni-Charged Resin FF GenScript Biotech Cat# L00666 SIM SF Expression Medium SinoBiological Cat# MSF1 DMEM Gibco Cat# 11965092 Characterized Fetal Bovine Serum (FBS), New Zealand Origin HyClone Cat# SH30406.05 HBSS, Calcium, magnesium Gibco Cat# 14025-092 Human CXCL12 PeproTech Cat# 30028A FLAG peptide GenScript Custom Synthesis Lipofectamine 3000 Invitrogen Cat# L3000001 Forskolin MCE Cat# HY-15371 Coelenterazine H Yeasen Biotech Cat# 40906ES Critical commercial assays ClonExpress Ultra One Step Cloning Kit Vazyme Biotech Cat# C115 Bac-to-Bac Expression System Invitrogen Cat# A11098 LANCE Ultra cAMP kit PerkinElmer Life Sciences Cat# TRF0263 Deposited data CXCL12-CXCR4-Gi complex EM map This work EMDB entry ID: EMD-36869 CXCL12-CXCR4-Gi complex coordinates This work PDB entry ID: 8K3Z Experimental models: Cell lines Spodoptera frugiperda Sf9 insect cell line Invitrogen Cat# 11496015 Trichoplusia ni Hi5 insect cell line Invitrogen Cat# B85502 HEK293T cell line ATCC Cat# CRL-3216 HeLa cell line ATCC Cat# CCL2 Recombinant DNA pFastBac-FLAG-CXCR4 This work N/A pFastBac-DN Gai This work N/A pFastBac-Gb1 This work N/A pFastBac-Gg2 This work N/A pFastBac-CXCL12 This work N/A pFastBac-gp67-scFv16 This work N/A pcDNA3.1-CXCR4 This work N/A pcDNA3.1-CXCR4(C28A) This work N/A pcDNA3.1-CXCR4(R134A) This work N/A (Continued on next page) Cell Reports 43, 114578, August 27, 2024 15 ..

    Protease Inhibitor:

    Article Title: Structural insights into ligand recognition and activation of the succinate receptor SUCNR1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC-labeled anti-FLAG antibody Sigma Cat# F4049; RRID: AB_439701 Bacterial and virus strains E. coli DH5a NEB Cat# C2988 Chemicals, peptides, and recombinant proteins Apyrase Sigma Cat# A6535 Protease Inhibitor Cocktail, EDTA-free TargetMol Cat# C0001 n-dodecyl-b-d-maltoside (DDM) Anatrace Cat# D310 Cholesteryl hemisuccinate (CHS) Anatrace Cat# CH210 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432 Lauryl maltose neopentyglycol (LMNG) Anatrace Cat# NG310 High Affinity Ni-Charged Resin FF GenScript Biotech Cat# L00666 SIM SF Expression Medium SinoBiological Cat# MSF1 DMEM Gibco Cat# 11965092 Characterized Fetal Bovine Serum (FBS), New Zealand Origin HyClone Cat# SH30406.05 HBSS, Calcium, magnesium Gibco Cat# 14025-092 Succinate Sangon Biotech Cat# A610496 cis-epoxysuccinate MCE Cat# HY-125791 FLAG peptide GenScript Custom Synthesis Lipofectamine 3000 Invitrogen Cat# L3000001 Forskolin MCE Cat# HY-15371 Coelenterazine H Yeasen Biotech Cat# 40906ES Critical commercial assays ClonExpress Ultra One Step Cloning Kit Vazyme Biotech Cat# C115 Bac-to-Bac Expression System Invitrogen Cat# A11098 LANCE Ultra cAMP kit PerkinElmer Life Sciences Cat# TRF0263 Deposited data succinate-SUCNR1-Gi complex EM map This work EMDB entry ID: EMD-37686 succinate-SUCNR1-Gi complex coordinates This work PDB entry ID: 8WOG epoxysuccinate-SUCNR1-Gi complex EM map This work EMDB entry ID: EMD-37707 epoxysuccinate-SUCNR1-Gi complex coordinates This work PDB entry ID: 8WP1 Experimental models: Cell lines Spodoptera frugiperda Sf9 insect cell line Invitrogen Cat# 11496015 Trichoplusia ni Hi5 insect cell line Invitrogen Cat# B85502 HEK293T cell line ATCC Cat# CRL-3216 HeLa cell line ATCC Cat# CCL2 Recombinant DNA pFastBac-FLAG-SUCNR1 This work N/A pFastBac-DN Gai This work N/A pFastBac-Gb1 This work N/A pFastBac-Gg2 This work N/A pcDNA3.1-SUCNR1 This work N/A pcDNA3.1-SUCNR1 (Y30A) This work N/A (Continued on next page) Cell Reports 43, 114381, July 23, 2024 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1-SUCNR1 (Y83A) This work N/A pcDNA3.1-SUCNR1 (R99A) This work N/A pcDNA3.1-SUCNR1 (F175A) This work N/A pcDNA3.1-SUCNR1 (Y248A) This work N/A pcDNA3.1-SUCNR1 (Y277A) This work N/A pcDNA3.1-SUCNR1 (R281A) This work N/A pcDNA3.1-Gai1-LgBiT This work N/A pcDNA3.1-Gb1 This work N/A pcDNA3.1-SmBiT-Gg2 This work N/A Software and algorithms cryoSPARC v3.3.1 Structura Biotechnology https://cryosparc.com/ Chimera UCSF https://www.cgl.ucsf.edu/chimera/ ChimeraX UCSF https://www.cgl.ucsf.edu/chimerax/ COOT Emsley et al., 201036 https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Phenix Liebschner et al., 201937 https://phenix-online.org/ CHARMM-GUI Lehigh University https://www.charmm-gui.org/ GROMACS Abraham et al., 201538 https://www.gromacs.org/ PyMOL Schrödinger https://pymol.org/2/ GraphPad Prism GraphPad https://www.graphpad.com/ Other Amicon Ultra Centrifugal Filters-100k Millipore Cat# UFC9100 Superose 6 Increase 10/300GL column GE Healthcare Cat# 29091596 Superdex 200 Increase 10/300 column GE Healthcare Cat# 28990944 Quantifoil Au 300 mesh R1.2/1.3 grids Sigma Cat# TEM-Q350AR1.3

    Article Title: Cryo-EM structure of monomeric CXCL12-bound CXCR4 in the active state.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APC-labeled anti-CXCR4 antibody Thermo Fisher Scientific Cat# 17-9999-42; RRID:AB_1724113 Bacterial and virus strains E. coli DH5a NEB Cat# C2988 Chemicals, peptides, and recombinant proteins Apyrase Sigma Cat# A6535 Protease Inhibitor Cocktail, EDTA-free TargetMol Cat# C0001 n-dodecyl-b-d-maltoside (DDM) Anatrace Cat# D310 Cholesteryl hemisuccinate (CHS) Anatrace Cat# CH210 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432 Lauryl maltose neopentyglycol (LMNG) Anatrace Cat# NG310 High Affinity Ni-Charged Resin FF GenScript Biotech Cat# L00666 SIM SF Expression Medium SinoBiological Cat# MSF1 DMEM Gibco Cat# 11965092 Characterized Fetal Bovine Serum (FBS), New Zealand Origin HyClone Cat# SH30406.05 HBSS, Calcium, magnesium Gibco Cat# 14025-092 Human CXCL12 PeproTech Cat# 30028A FLAG peptide GenScript Custom Synthesis Lipofectamine 3000 Invitrogen Cat# L3000001 Forskolin MCE Cat# HY-15371 Coelenterazine H Yeasen Biotech Cat# 40906ES Critical commercial assays ClonExpress Ultra One Step Cloning Kit Vazyme Biotech Cat# C115 Bac-to-Bac Expression System Invitrogen Cat# A11098 LANCE Ultra cAMP kit PerkinElmer Life Sciences Cat# TRF0263 Deposited data CXCL12-CXCR4-Gi complex EM map This work EMDB entry ID: EMD-36869 CXCL12-CXCR4-Gi complex coordinates This work PDB entry ID: 8K3Z Experimental models: Cell lines Spodoptera frugiperda Sf9 insect cell line Invitrogen Cat# 11496015 Trichoplusia ni Hi5 insect cell line Invitrogen Cat# B85502 HEK293T cell line ATCC Cat# CRL-3216 HeLa cell line ATCC Cat# CCL2 Recombinant DNA pFastBac-FLAG-CXCR4 This work N/A pFastBac-DN Gai This work N/A pFastBac-Gb1 This work N/A pFastBac-Gg2 This work N/A pFastBac-CXCL12 This work N/A pFastBac-gp67-scFv16 This work N/A pcDNA3.1-CXCR4 This work N/A pcDNA3.1-CXCR4(C28A) This work N/A pcDNA3.1-CXCR4(R134A) This work N/A (Continued on next page) Cell Reports 43, 114578, August 27, 2024 15 ..

    Expressing:

    Article Title: Structural insights into ligand recognition and activation of the succinate receptor SUCNR1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC-labeled anti-FLAG antibody Sigma Cat# F4049; RRID: AB_439701 Bacterial and virus strains E. coli DH5a NEB Cat# C2988 Chemicals, peptides, and recombinant proteins Apyrase Sigma Cat# A6535 Protease Inhibitor Cocktail, EDTA-free TargetMol Cat# C0001 n-dodecyl-b-d-maltoside (DDM) Anatrace Cat# D310 Cholesteryl hemisuccinate (CHS) Anatrace Cat# CH210 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432 Lauryl maltose neopentyglycol (LMNG) Anatrace Cat# NG310 High Affinity Ni-Charged Resin FF GenScript Biotech Cat# L00666 SIM SF Expression Medium SinoBiological Cat# MSF1 DMEM Gibco Cat# 11965092 Characterized Fetal Bovine Serum (FBS), New Zealand Origin HyClone Cat# SH30406.05 HBSS, Calcium, magnesium Gibco Cat# 14025-092 Succinate Sangon Biotech Cat# A610496 cis-epoxysuccinate MCE Cat# HY-125791 FLAG peptide GenScript Custom Synthesis Lipofectamine 3000 Invitrogen Cat# L3000001 Forskolin MCE Cat# HY-15371 Coelenterazine H Yeasen Biotech Cat# 40906ES Critical commercial assays ClonExpress Ultra One Step Cloning Kit Vazyme Biotech Cat# C115 Bac-to-Bac Expression System Invitrogen Cat# A11098 LANCE Ultra cAMP kit PerkinElmer Life Sciences Cat# TRF0263 Deposited data succinate-SUCNR1-Gi complex EM map This work EMDB entry ID: EMD-37686 succinate-SUCNR1-Gi complex coordinates This work PDB entry ID: 8WOG epoxysuccinate-SUCNR1-Gi complex EM map This work EMDB entry ID: EMD-37707 epoxysuccinate-SUCNR1-Gi complex coordinates This work PDB entry ID: 8WP1 Experimental models: Cell lines Spodoptera frugiperda Sf9 insect cell line Invitrogen Cat# 11496015 Trichoplusia ni Hi5 insect cell line Invitrogen Cat# B85502 HEK293T cell line ATCC Cat# CRL-3216 HeLa cell line ATCC Cat# CCL2 Recombinant DNA pFastBac-FLAG-SUCNR1 This work N/A pFastBac-DN Gai This work N/A pFastBac-Gb1 This work N/A pFastBac-Gg2 This work N/A pcDNA3.1-SUCNR1 This work N/A pcDNA3.1-SUCNR1 (Y30A) This work N/A (Continued on next page) Cell Reports 43, 114381, July 23, 2024 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1-SUCNR1 (Y83A) This work N/A pcDNA3.1-SUCNR1 (R99A) This work N/A pcDNA3.1-SUCNR1 (F175A) This work N/A pcDNA3.1-SUCNR1 (Y248A) This work N/A pcDNA3.1-SUCNR1 (Y277A) This work N/A pcDNA3.1-SUCNR1 (R281A) This work N/A pcDNA3.1-Gai1-LgBiT This work N/A pcDNA3.1-Gb1 This work N/A pcDNA3.1-SmBiT-Gg2 This work N/A Software and algorithms cryoSPARC v3.3.1 Structura Biotechnology https://cryosparc.com/ Chimera UCSF https://www.cgl.ucsf.edu/chimera/ ChimeraX UCSF https://www.cgl.ucsf.edu/chimerax/ COOT Emsley et al., 201036 https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Phenix Liebschner et al., 201937 https://phenix-online.org/ CHARMM-GUI Lehigh University https://www.charmm-gui.org/ GROMACS Abraham et al., 201538 https://www.gromacs.org/ PyMOL Schrödinger https://pymol.org/2/ GraphPad Prism GraphPad https://www.graphpad.com/ Other Amicon Ultra Centrifugal Filters-100k Millipore Cat# UFC9100 Superose 6 Increase 10/300GL column GE Healthcare Cat# 29091596 Superdex 200 Increase 10/300 column GE Healthcare Cat# 28990944 Quantifoil Au 300 mesh R1.2/1.3 grids Sigma Cat# TEM-Q350AR1.3

    Article Title: Cryo-EM structure of monomeric CXCL12-bound CXCR4 in the active state.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APC-labeled anti-CXCR4 antibody Thermo Fisher Scientific Cat# 17-9999-42; RRID:AB_1724113 Bacterial and virus strains E. coli DH5a NEB Cat# C2988 Chemicals, peptides, and recombinant proteins Apyrase Sigma Cat# A6535 Protease Inhibitor Cocktail, EDTA-free TargetMol Cat# C0001 n-dodecyl-b-d-maltoside (DDM) Anatrace Cat# D310 Cholesteryl hemisuccinate (CHS) Anatrace Cat# CH210 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432 Lauryl maltose neopentyglycol (LMNG) Anatrace Cat# NG310 High Affinity Ni-Charged Resin FF GenScript Biotech Cat# L00666 SIM SF Expression Medium SinoBiological Cat# MSF1 DMEM Gibco Cat# 11965092 Characterized Fetal Bovine Serum (FBS), New Zealand Origin HyClone Cat# SH30406.05 HBSS, Calcium, magnesium Gibco Cat# 14025-092 Human CXCL12 PeproTech Cat# 30028A FLAG peptide GenScript Custom Synthesis Lipofectamine 3000 Invitrogen Cat# L3000001 Forskolin MCE Cat# HY-15371 Coelenterazine H Yeasen Biotech Cat# 40906ES Critical commercial assays ClonExpress Ultra One Step Cloning Kit Vazyme Biotech Cat# C115 Bac-to-Bac Expression System Invitrogen Cat# A11098 LANCE Ultra cAMP kit PerkinElmer Life Sciences Cat# TRF0263 Deposited data CXCL12-CXCR4-Gi complex EM map This work EMDB entry ID: EMD-36869 CXCL12-CXCR4-Gi complex coordinates This work PDB entry ID: 8K3Z Experimental models: Cell lines Spodoptera frugiperda Sf9 insect cell line Invitrogen Cat# 11496015 Trichoplusia ni Hi5 insect cell line Invitrogen Cat# B85502 HEK293T cell line ATCC Cat# CRL-3216 HeLa cell line ATCC Cat# CCL2 Recombinant DNA pFastBac-FLAG-CXCR4 This work N/A pFastBac-DN Gai This work N/A pFastBac-Gb1 This work N/A pFastBac-Gg2 This work N/A pFastBac-CXCL12 This work N/A pFastBac-gp67-scFv16 This work N/A pcDNA3.1-CXCR4 This work N/A pcDNA3.1-CXCR4(C28A) This work N/A pcDNA3.1-CXCR4(R134A) This work N/A (Continued on next page) Cell Reports 43, 114578, August 27, 2024 15 ..

    Cloning:

    Article Title: Structural insights into ligand recognition and activation of the succinate receptor SUCNR1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC-labeled anti-FLAG antibody Sigma Cat# F4049; RRID: AB_439701 Bacterial and virus strains E. coli DH5a NEB Cat# C2988 Chemicals, peptides, and recombinant proteins Apyrase Sigma Cat# A6535 Protease Inhibitor Cocktail, EDTA-free TargetMol Cat# C0001 n-dodecyl-b-d-maltoside (DDM) Anatrace Cat# D310 Cholesteryl hemisuccinate (CHS) Anatrace Cat# CH210 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432 Lauryl maltose neopentyglycol (LMNG) Anatrace Cat# NG310 High Affinity Ni-Charged Resin FF GenScript Biotech Cat# L00666 SIM SF Expression Medium SinoBiological Cat# MSF1 DMEM Gibco Cat# 11965092 Characterized Fetal Bovine Serum (FBS), New Zealand Origin HyClone Cat# SH30406.05 HBSS, Calcium, magnesium Gibco Cat# 14025-092 Succinate Sangon Biotech Cat# A610496 cis-epoxysuccinate MCE Cat# HY-125791 FLAG peptide GenScript Custom Synthesis Lipofectamine 3000 Invitrogen Cat# L3000001 Forskolin MCE Cat# HY-15371 Coelenterazine H Yeasen Biotech Cat# 40906ES Critical commercial assays ClonExpress Ultra One Step Cloning Kit Vazyme Biotech Cat# C115 Bac-to-Bac Expression System Invitrogen Cat# A11098 LANCE Ultra cAMP kit PerkinElmer Life Sciences Cat# TRF0263 Deposited data succinate-SUCNR1-Gi complex EM map This work EMDB entry ID: EMD-37686 succinate-SUCNR1-Gi complex coordinates This work PDB entry ID: 8WOG epoxysuccinate-SUCNR1-Gi complex EM map This work EMDB entry ID: EMD-37707 epoxysuccinate-SUCNR1-Gi complex coordinates This work PDB entry ID: 8WP1 Experimental models: Cell lines Spodoptera frugiperda Sf9 insect cell line Invitrogen Cat# 11496015 Trichoplusia ni Hi5 insect cell line Invitrogen Cat# B85502 HEK293T cell line ATCC Cat# CRL-3216 HeLa cell line ATCC Cat# CCL2 Recombinant DNA pFastBac-FLAG-SUCNR1 This work N/A pFastBac-DN Gai This work N/A pFastBac-Gb1 This work N/A pFastBac-Gg2 This work N/A pcDNA3.1-SUCNR1 This work N/A pcDNA3.1-SUCNR1 (Y30A) This work N/A (Continued on next page) Cell Reports 43, 114381, July 23, 2024 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1-SUCNR1 (Y83A) This work N/A pcDNA3.1-SUCNR1 (R99A) This work N/A pcDNA3.1-SUCNR1 (F175A) This work N/A pcDNA3.1-SUCNR1 (Y248A) This work N/A pcDNA3.1-SUCNR1 (Y277A) This work N/A pcDNA3.1-SUCNR1 (R281A) This work N/A pcDNA3.1-Gai1-LgBiT This work N/A pcDNA3.1-Gb1 This work N/A pcDNA3.1-SmBiT-Gg2 This work N/A Software and algorithms cryoSPARC v3.3.1 Structura Biotechnology https://cryosparc.com/ Chimera UCSF https://www.cgl.ucsf.edu/chimera/ ChimeraX UCSF https://www.cgl.ucsf.edu/chimerax/ COOT Emsley et al., 201036 https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Phenix Liebschner et al., 201937 https://phenix-online.org/ CHARMM-GUI Lehigh University https://www.charmm-gui.org/ GROMACS Abraham et al., 201538 https://www.gromacs.org/ PyMOL Schrödinger https://pymol.org/2/ GraphPad Prism GraphPad https://www.graphpad.com/ Other Amicon Ultra Centrifugal Filters-100k Millipore Cat# UFC9100 Superose 6 Increase 10/300GL column GE Healthcare Cat# 29091596 Superdex 200 Increase 10/300 column GE Healthcare Cat# 28990944 Quantifoil Au 300 mesh R1.2/1.3 grids Sigma Cat# TEM-Q350AR1.3

    Article Title: Cryo-EM structure of monomeric CXCL12-bound CXCR4 in the active state.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APC-labeled anti-CXCR4 antibody Thermo Fisher Scientific Cat# 17-9999-42; RRID:AB_1724113 Bacterial and virus strains E. coli DH5a NEB Cat# C2988 Chemicals, peptides, and recombinant proteins Apyrase Sigma Cat# A6535 Protease Inhibitor Cocktail, EDTA-free TargetMol Cat# C0001 n-dodecyl-b-d-maltoside (DDM) Anatrace Cat# D310 Cholesteryl hemisuccinate (CHS) Anatrace Cat# CH210 Anti-DYKDDDDK G1 Affinity Resin GenScript Biotech Cat# L00432 Lauryl maltose neopentyglycol (LMNG) Anatrace Cat# NG310 High Affinity Ni-Charged Resin FF GenScript Biotech Cat# L00666 SIM SF Expression Medium SinoBiological Cat# MSF1 DMEM Gibco Cat# 11965092 Characterized Fetal Bovine Serum (FBS), New Zealand Origin HyClone Cat# SH30406.05 HBSS, Calcium, magnesium Gibco Cat# 14025-092 Human CXCL12 PeproTech Cat# 30028A FLAG peptide GenScript Custom Synthesis Lipofectamine 3000 Invitrogen Cat# L3000001 Forskolin MCE Cat# HY-15371 Coelenterazine H Yeasen Biotech Cat# 40906ES Critical commercial assays ClonExpress Ultra One Step Cloning Kit Vazyme Biotech Cat# C115 Bac-to-Bac Expression System Invitrogen Cat# A11098 LANCE Ultra cAMP kit PerkinElmer Life Sciences Cat# TRF0263 Deposited data CXCL12-CXCR4-Gi complex EM map This work EMDB entry ID: EMD-36869 CXCL12-CXCR4-Gi complex coordinates This work PDB entry ID: 8K3Z Experimental models: Cell lines Spodoptera frugiperda Sf9 insect cell line Invitrogen Cat# 11496015 Trichoplusia ni Hi5 insect cell line Invitrogen Cat# B85502 HEK293T cell line ATCC Cat# CRL-3216 HeLa cell line ATCC Cat# CCL2 Recombinant DNA pFastBac-FLAG-CXCR4 This work N/A pFastBac-DN Gai This work N/A pFastBac-Gb1 This work N/A pFastBac-Gg2 This work N/A pFastBac-CXCL12 This work N/A pFastBac-gp67-scFv16 This work N/A pcDNA3.1-CXCR4 This work N/A pcDNA3.1-CXCR4(C28A) This work N/A pcDNA3.1-CXCR4(R134A) This work N/A (Continued on next page) Cell Reports 43, 114578, August 27, 2024 15 ..



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